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Unexpected elution behaviour of peptides with reversed phase columns
Summary
Type:
HPLC
Application #:
107970
Catalog#:
720051.40 - MN HPLC Column, Nucleosil C18, 120A, 5um, 4x125mm
Detection:
UV, 220nm
Conditions:
eluent: gradient from 28% aqueous acetonitrile, 0.1% trifluoroacetic acid (TFA) to 40% acetonitrile, 0.1% TFA in 30min
Authors:
Engelhardt H
Source:
J Chromatogr 499 (1990) 165 - 175
Details
Legend: Fig. 4. Separation of standard peptides, 1. CCK8 sulphated, 2. CCK8 desulphated, 3. gastrin-17 sulphated, 4. gastrin-17 desulphated.
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